ISBN: 978-981-09-5471-0 DOI: 10.18178/wcse.2015.04.074
Mechanical Analysis and Experimental Research on Adjusting Technique for Cellular Position and Attitude in Microinjectio
Abstract— In microinjection, Cell position and attitude adjusting is the key step, which greatly
affects the experimental result. However, based on laser, electric field, and magnetic field etc., the
techniques have many disadvantages such as high damage, complex adjusting process, and low
precision and efficiency. On micro scale, this paper analyses demand and principle of cell position
and attitude adjusting and then proposes an automatic adjusting method. With end-actuator of
holding micropipette, the cell position and attitude adjusting technique makes adjusting motion
decompose into rotary motion of two orthogonal planes. Therefore, cell position and attitude
adjusting system is developed. According to attitude angle of cell reference point calculated by
image retrieval system, position and attitude of cell is adjusted. With the above technique, shrimp
egg position and attitude adjusting is achieved. The result shows that the technique simplifies
adjusting processing and improves the stability of processing. Additionally, the adjusted cell
position and attitude meets the demand of automatic positing in cell microinjection.
Index Terms— Micromanipulation; Cell position and attitude adjusting; Mechanical movement; Digital
image processing
Ying Wang, Xinyan Huang, Li Zhu, Hongcheng Wang
School of Mechanical Engineering, Nanjing University of Science and Technology, CHINA
Cite: Ying Wang, Xinyan Huang, Li Zhu, Hongcheng Wang, "Mechanical Analysis and Experimental Research on Adjusting Technique for Cellular Position and Attitude in Microinjection," 2015 The 5th International Workshop on Computer Science and Engineering-Information Processing and Control Engineering (WCSE 2015-IPCE), pp. 449-454, Moscow, Russia, April 15-17, 2015.